In this study, effective parameters on pseudomonas bacterial culturing are analyzed individually. Pseudomonas bacteria were applied for oil pollutants segregation from a polluted water sample of Persian Gulf. The bacteria were used for cleaning the sample so that the contents’ chemical features could be determined.Salinity and pH parameters were put under observation in different conditions and the results are illustrated in diagrams. Concentration of biosurfactants and conditions of culture medium like oxygen amount and temperature were all determined and analyzed. The influencing parameters on laboratory scale bacterial culturing were varied individually in order to obtain the optimum conditions for oil pollutants cleaning.Taguchi experimental design method was then applied to optimize the bacterial culturing conditions. Salinity, pH amounts, and biosurfactants concentrations were chosen as the parameters that use different levels based on Taguchi method. Eventually, a pH of 8.5, salinity of %2.5 and biosurfactants concentration of %0.1 were determined as the optimum conditions in which bacteria were cultured and grown the most.
Key words: culture media, bacteria, biosurfactants, salinity, Persian Gulf, crude oil